Pdf portable document format files that can be viewed on any. Indicator lights power switch alarm a check the indicator lights to see if the waste is full or the sheath is empty. Bd biosciences recommends that firsttime users take advantage of operator training offered with the sale of every new instrument. Reading this material and answering the questions at the. Bd facscanto ii instructions for use manual pdf download. Documents document view page bd facscalibur cytometer manual.
If the fluidics need to be changed, please seek guidance from. This instrument was one of the first mass produced flow cytometers. Designed specifically to support a wide range of applications, the bd facs calibur system is a. Comparison and evaluation of seven different bench. An electrical pulse the voltage pulse is generated and is processed by the signal processilti fthing electronics of the flow cytometer. Facs200 handbook pro 1 facscalibur facs200 handbook pro dna facscalibur 1. Bd facscalibur flow cytometry system bd biosciences. The facscalibur is still prevalent in many labs around the world. The bd facscalibur flow cytometer is another bd biosciences first. A to perform the steps in this tutorial, you will need. These instruments include a bectondickinson bd facsvantagese high speed cell sorter, and a bd facscalibur bench top flow cytometer. Bd facscalibur instructions for use bd biosciences.
The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of. Guide to using bd cba kits with the bd facscalibur system. Flow cytometer facscalibur and facsvantage flow sorter. Flow cytometry service bd facscalibur how to use guide 5 2 singlestained samples for every fluorochrome to be analyzed in the cytometer menu, open the detectorsamps and compensation panels. Umms flow cytometry core laboratory storage of data. The bd facscalibur system consists of a flow cytometer and bd facstation. Microscopy and microanalysis facility university of new brunswick. For lw and lnw assays, bd facscomp software automatically adjusts pmt voltages and fluorescence compensation, and evaluates instrument sensitivity using bd calibrite beads. Bd facscalibur flow cytometry system technical specifications for research use only. Aug 10, 2017 bd facsverse flow cytometer is available from the lab world group. Untitledn the software adds a number to each subsequent file. Bd facsverse flow cytometer is available from the lab world group.
The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis through modular components. The flow cytometer is used to measure physical and chemical properties of cells or particles in suspension as they flow, in single file, through a beam of light. Bd facscalibur bd pdf catalogs technical documentation. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. Introduction the bd facscalibur flow cytometer identifies and enumerates lymphocyte subsets in human cells in suspension. Bd facscalibur, bd facscan, bd facsort, or bd lsr flow cytometer. This information can be used to individually sort or separate subpopulations of cells. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the bd facscalibur. For applicatio nspecific instructions, refer to the individual application guides and information supplied with the reagents. Bd facs calibur microscopy and microanalysis facility. Jun 07, 2006 the bd facscanto is often hailed as the new generation of flow cytometry.
Bd unveils new bd facscelesta flow cytometer during the 2015 american society for cell biology annual meeting. Flow cytometry flow cytometry is the technical process that allows for the individual measurements of cell fluorescence and light scattering. Bd biosciences accuri c6 personal flow cytometer features. Reading this material and answering the questions at the end of each. The gold standard that continues to evolve the bd facscalibur flow cytometer is another bd biosciences first. Bd announces results for 2020 first fiscal quarter. Not for use in diagnostic or therapeutic procedures.
Other applications flow cytometry is indicated in the evaluation of serous effusions and csf, including aqueous or vitreous humor of patients with a history of hematolymphoid neoplasia. The bd facscanto flow cytometer reference manual assumes you have a. Bd biosciences recommends that firsttime users of this instrument take advantage of operator training offered with the sale of every new instrument. How to operate the bd facscanto flow cytometer preface dear colleague, the bd facscanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells. An optional, auto mated sample loader is also available. Fl4 adjustments apply only to a bd facscalibur or bd facsort flow cytometer equipped with the fl4 option. While only a four color, six parameter analog system, this machine is stable and rarely requires service. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. A cellquest users guide pdf is on the computer desktop feel free to copy it onto a usb drive if. Optical path configuration flow cytometry has evolved rapidly to incorporate the use of multiple fluorochromes to effectively identify and isolate subset populations. It is a tricolor, automated system that is capable of analyzing a wide variety of. Bd, the bd logo, bd calibrite, bd cellquest, bd clonecyt, bd facs, bd facscalibur, bd.
To assess substantial equivalency, the aquios cl flow cytometer with aquios tetra reagents was compared against the bd facscalibur flow cytometer with bd multitest reagents using a single platform approach for absolute counts. Fitc and alexa fluor 488 fl1 channelpe fl2percp, pecy5, percpcy5. A type of flow cytometer manufactured and sold by bd biosciences. A cellquest users guide pdf is on the computer desktop feel free to copy it onto a. Flow cytometer signal detection as a ppp garticle passes through the laser and fluoresces, it is detected by a photodetector pmt. Becton dickinson facscalibur flow cytometry system youtube. For more information, please visit us at bd facsverseflo.
Bd facs canto ii flow cytometer quick user guide v. Jan 09, 2006 the facscalibur system is a modular, benchtop flow cytometer from becton dickinson immunocytometry system bdis that is easily modified based on research needs. The facsvantagese will sort or isolate subpopulations of cells from a. Turn on the facsflow supply system located under the bench green switch on right side.
Bd facsdiva software features specific to the bd facscanto cytometer within this manual. Flow cytometer components figure 11 bd facscanto ii flow cytometer flow cell access door side door data ports optics access door acquisition indicator lights sample injection tube power button fluidics cart connections do not place heavy objects on top of the cytometer at any time. Facs and falcon are registered trademarks of becton dickinson and company. Read the section of the manual specific to the instrument operation that you. Turn on flow cytometer by pushing green button on right side of. Cell flow instruments and the technical support and expertise will be available to the lbnl and external research community. For more information about the bd facscalibur system and other flow cytometry products from bd biosciences, visit. Bd facscalibur system is fully modular so it can be upgraded to meet future needs.
Bd accuri c6 system 32bit software is not forward compatible with bd accuri c6 plus 64bit software and the fcs 3. Becton dickinson facscalibur flow cytometry system with hts autosampler available at the lab world group. Bd facscalibur flow cytometer from bd biosciences selectscience. It designed for applications that range from routine clinical to advanced research, analyzing single cells as they pass through a focused laser beam. This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day. All reagents and kits are compatible with both bd accuri c6 plus and accuri c6 flow cytometer systems.
Designed specifically to support a wide range of applications, the. Dont grossly overbook since we have to pay for both reserved time and the time actually used which ever is greater. An optional, automated sample loader is also available. The bd facscalibur platform allows users to perform both cell analysis and cell sorting in a single benchtop system. Bd facscalibur the flow cytometer for your routine cell analysis needs. Anyone have any experience with this problemvolunteer a solution.
Designed specifically to support a wide range of applications, the bd facscalibur system offers easy to use software, multi sample loading options, and. The intuitive bd facscalibur flow cytometer instrument can be upgraded to keep up with the laboratorys sorting and automation needs. The bd facscalibur flow cytometry system is another bd first. Dec 03, 2014 flow cytometry is a rapid and useful method of obtaining a diagnosis. A multicenter study was conducted to target the intended use population following clsi ep09a3.
Bd facscalibur system offers easy to use software, multisample loading options, and. One striking difference between the facscanto and older generation machines such as the facscalibur is that this system operates on a pcbased windows xp platform using bd facsdiva software. The bd facscalibur instructions for use assumes you have a working knowledge of the mac os x operating system. The bd facscanto clinical software reference manual assumes you have a working knowledge of basic microsoft windows operation. This process is performed at rates of thousands of cells per second. Bd facscalibur instructions for use manual pdf download. Conventions the following conventions are used in these instructions. As a modular option for new or existing bd facscalibur flow cytometers, the hts option is designed to speed through a variety of microtiter plates in less than 15 minutes and support a wide range of applications including the bd cytometric bead array assay. Bectondickinson facscalibur flow cytometer instructions. A growing number of flow cytometrybased assays depend on.